Effects of three inhibitors of nitric oxide synthase on host resistance to bacterial infection
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K Fukatsu1, H Saito, R Fukushima
1Department of Surgery, University of Tokyo, Japan.
Objective:
To examine the effects of three nitric oxide synthase inhibitors on survival in a murine sepsis model.
Design:
Prospective randomized experimental trials.
Setting:
Laboratory.
Subjects:
Female Balb/c mice.
Interventions:
Escherichia coli (10(8) colony-forming-units/body) were injected into the peritoneal cavities of Balb/c mice. NG-nitro-L-arginine-methyl-ester, NG-monomethyl-L-arginine, or N-iminoethyl-L-ornithine was given at various concentrations, intraperitoneally, one hour before bacterial challenge.
Measurements:
One hundred and fifteen animals were observed for survival.
Results:
These inhibitors provided the mice no protection from the bacterial challenge. Notably, pretreatment with NG-nitro-L-arginine-methyl-ester (100 mg/kg i.p.) actually reduced survival time after E. coli challenge.
Conclusions:
Inhibition of nitric oxide production improved neither the survival time nor rate in this murine sepsis model.