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Updated: Aug 11, 2026

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Dyeing Insects for Behavioral Assays: the Mating Behavior of Anesthetized Drosophila
Published on: April 22, 2015
Effects of caffeine on mating frequency and pre-copulation and copulation durations in Drosophila prosaltans
M M Itoyama1, H E de Campos Bicudo, A J Manzato
1Departamento de Ciências Naturais, Centro Universitário de Três Lagoas, Universidade Federal de Mato Grosso do Sul, Brazil.
Abstract:
Parameters of sexual behaviour were studied in Drosophila prosaltans treated with 2,500 micrograms/ml of caffeine per 1 ml of banana culture medium. The mating frequency and copulation duration were greater in control than in treated flies, while the pre-copulation duration was greater in treated flies than in controls. Statistical analysis showed that for the pre-copulation duration the difference was significant.

