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Lineage commitment of CD34+ human hematopoietic progenitor cells
G E Tjønnfjord1, R Steen, O P Veiby
1Institute of Transplantation Immunology, The National Hospital, University of Oslo, Norway.
Experimental Hematology
|July 1, 1996
Summary
Surface molecule expression on hematopoietic progenitor cells does not always indicate lineage commitment. However, CD13 expression suggests myeloid development, and CD20 expression is specific to B lymphocyte progenitors.
Area of Science:
- Hematology
- Immunology
- Cell Biology
Background:
- Hematopoietic cells differentiate along a continuum from stem cells to lineage-specific cells.
- Progenitor cell commitment to a specific lineage is a critical developmental step.
- Surface molecules distinguishing multipotential from lineage-committed progenitor cells are largely unknown.
Purpose of the Study:
- To identify surface molecules that indicate lineage commitment in hematopoietic progenitor cells.
- To investigate the role of specific cell surface markers in early hematopoiesis.
Main Methods:
- Utilized a thymic stromal cell co-culture system.
- Analyzed CD34+ bone marrow cells expressing various surface markers (CD2, CD7, CD10, CD19, CD33, CD13, CD14, CD15, CD20).
- Assessed the differentiation potential of these progenitor populations.
Main Results:
- Expression of CD2, CD7, CD10, CD19, or CD33 on CD34+ cells did not preclude differentiation into T lymphocytes, granulocytes, or monocytes.
- CD34+CD13bright, CD34+CD14+, and CD34+CD15+ cells yielded myeloid progeny.
- CD34+CD20+ cells did not differentiate into T lymphoid or myeloid lineages.
Conclusions:
- CD13 expression appears to coincide with commitment to myeloid development, preceding CD14 and CD15.
- CD20 expression is restricted to progenitor cells committed to B lymphocyte development.
- Certain surface markers on progenitor cells do not signify irreversible lineage commitment.