Variations in microsatellite sequences provide evidence for population differences and multiple ribosomal gene...

Enzymatic amplification of expansion segment 5 sequences within domain IV of the large subunit ribosomal DNA generated distinct results among geographical isolates of Trichinella pseudospiralis from Russia, North America, and Australia from both avian and mammalian hosts. Discrete, multiple DNA fragments ranging in approximate size from 285 to 360 bp were observed within and among each of the isolates tested. Polymerase chain reaction performed on individual adult parasites from each isolate resulted in multiple DNA fragments that were comparable to those generated from pooled genomic DNA. Sequence analysis of cloned, representative amplified fragments demonstrated that fragment length variation resulted primarily from the dinucleotide (TG)n and trinucleotide (TGC)n microsatellite repeats present within the expansion segment. Results are consistent with both population differences within the species as well as the presence of multiple alleles of the large subunit ribosomal RNA genes within individual parasites.