Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Isoenzyme]

H Ohta1, H Watanabe, N Sawabu

  • 1Department of Internal Medicine, Tonami General Hospital, Kanazawa University.

Nihon Rinsho. Japanese Journal of Clinical Medicine
|June 1, 1996
PubMed
Summary
This summary is machine-generated.

Cancerous isoenzymes like alkaline phosphatase (ALP) and gamma-glutamyltranspeptidase (gamma-GTP) can aid in diagnosing various cancers. A novel gamma-GTP isoenzyme shows high specificity for detecting hepatocellular carcinoma (HCC).

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cloning, subcellular localization and expression of CHL1, a subunit of magnesium-chelatase in soybean.

Biochemical and biophysical research communications·1995
Same author

[A resected case of focal nodular hyperplasia of the liver which could be diagnosed before operation].

Nihon Shokakibyo Gakkai zasshi = The Japanese journal of gastro-enterology·1995
Same author

Molecular characterization of fusion regulatory protein-1 (FRP-1) that induces multinucleated giant cell formation of monocytes and HIV gp160-mediated cell fusion. FRP-1 and 4F2/CD98 are identical molecules.

Journal of immunology (Baltimore, Md. : 1950)·1995
Same author

[Evaluation of early colorectal cancer with submucosal invasion based on histological architecture].

Nihon Shokakibyo Gakkai zasshi = The Japanese journal of gastro-enterology·1995
Same author

CT-defined large subcortical infarcts: correlation of location with site of cerebrovascular occlusive disease.

AJNR. American journal of neuroradiology·1995
Same author

[Pharmacological and biochemical investigation of intracavital fluorinated pyrimidine chemotherapy].

Gan to kagaku ryoho. Cancer & chemotherapy·1995
Same journal

[Development of novel therapeutics for multiple myeloma and improvement of drug lag].

Nihon rinsho. Japanese journal of clinical medicine·2019
Same journal

[Clinical pharmacy services to patients of immunomodulatory drugs].

Nihon rinsho. Japanese journal of clinical medicine·2019
Same journal

[Therapeutic drug monitoring of the new anti-myeloma drugs in the treatment of multiple myeloma].

Nihon rinsho. Japanese journal of clinical medicine·2019
Same journal

[Prognostic value of minimal residual disease assessment using next-generation sequencing in multiple myeloma].

Nihon rinsho. Japanese journal of clinical medicine·2019
Same journal

[The evaluation of minimal residual disease in multiple myeloma by an allele-specific oligonucleotide real-time PCR].

Nihon rinsho. Japanese journal of clinical medicine·2019
Same journal

[Evaluation of minimal residual disease in myeloma by multiparametric flow cytometry].

Nihon rinsho. Japanese journal of clinical medicine·2019
See all related articles

Area of Science:

  • Biochemistry
  • Oncology
  • Clinical Diagnostics

Context:

  • Cancerous isoenzymes, including alkaline phosphatase (ALP) and gamma-glutamyltranspeptidase (gamma-GTP), are expressed in various malignant tumors.
  • Specific isoenzymes like placental ALP and variant ALP are detected in patient sera, with variant ALP being a marker for hepatocellular carcinoma (HCC).
  • While ALP isoenzymes have limited diagnostic utility due to low positive rates (except in seminoma), a novel gamma-GTP isoenzyme shows promise.

Purpose:

  • To investigate the diagnostic potential of cancerous isoenzymes in various malignant tumors.
  • To evaluate the specificity of a novel gamma-glutamyltranspeptidase (gamma-GTP) isoenzyme for hepatocellular carcinoma (HCC) detection.

Summary:

  • Expression of cancerous isoenzymes has been studied using biochemical techniques.

Related Experiment Videos

  • Variant ALP is a specific tumor marker for HCC, though its detection rate is low.
  • A novel gamma-GTP isoenzyme is found in approximately 60% of HCC patient sera, compared to only 3% in benign liver diseases, indicating high specificity for HCC.

    • Impact:

    • Cancerous isoenzymes can serve as valuable biomarkers for the diagnosis of various malignant tumors.
    • The novel gamma-GTP isoenzyme offers a highly specific marker for the early detection and diagnosis of HCC.
    • This research highlights the potential of isoenzyme analysis in improving cancer diagnostics.