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Parenteral strobilar development of Echinococcus multilocularis in scid mice
J Inohara1, M C Playford, N Nonaka
1Department of Animal Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
The Japanese Journal of Veterinary Research
|May 1, 1996
Summary
Severe combined immuno-deficient (scid) mice allow Echinococcus multilocularis development outside the intestine. Parenteral infection in scid mice led to significant worm viability and reproductive organ development.
Area of Science:
- Parasitology
- Immunology
- Helminthology
Background:
- Echinococcus multilocularis is an intestinal parasite.
- Parenteral infection typically results in limited parasite development.
Purpose of the Study:
- To investigate Echinococcus multilocularis development in a parenteral environment.
- To assess the role of severe combined immuno-deficient (scid) mice in parasite development.
Main Methods:
- Inoculation of Echinococcus multilocularis protoscoleces into intracerebral, subcutaneous, and intraperitoneal sites in scid mice.
- Comparison of worm viability and development in scid mice versus immunologically normal C.B-17 control mice.
- Assessment of reproductive organ development after transplantation into golden hamsters.
Main Results:
- Parenteral strobilation and segmented worms were observed in scid mice at all inoculation sites.
- Worm viability was significantly higher in scid mice (69.1-91.4%) compared to control mice (4.0-48.0%) in subcutaneous and peritoneal sites.
- Worms from scid mice showed further reproductive organ development upon transplantation, indicating successful maturation.
Conclusions:
- The parenteral environment of scid mice supports the adult development of Echinococcus multilocularis.
- Immunodeficiency in scid mice facilitates parasite development outside the typical intestinal niche.
- This study highlights the potential for parenteral Echinococcus multilocularis development in immunocompromised hosts.