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Related Experiment Videos

Correlation between brain nitric oxide synthase activity and opiate withdrawal

J C Leza1, I Lizasoain, B Cuéllar

  • 1Departamento de Farmacologia, Facultad de Medicina, Universidad Complutense de Madrid, Spain.

Naunyn-Schmiedeberg'S Archives of Pharmacology
|February 1, 1996
PubMed
Summary

Opiate withdrawal in mice involves increased nitric oxide synthase (NOS) activity. Inhibiting NOS with L-NAME reduced withdrawal symptoms, suggesting NOS inhibitors may treat opiate withdrawal.

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Area of Science:

  • Neuroscience
  • Pharmacology

Background:

  • Opiate withdrawal is a complex physiological response.
  • Nitric oxide synthase (NOS) activity is implicated in neurological processes.

Purpose of the Study:

  • To investigate the role of nitric oxide synthase (NOS) in opiate withdrawal.
  • To explore the therapeutic potential of NOS inhibitors for opiate withdrawal.

Main Methods:

  • Morphine-dependent mice were administered naloxone to induce withdrawal.
  • Mice were treated with L-NAME, a NOS inhibitor, or saline.
  • Calcium-dependent NOS activity in the cerebellum was measured.
  • Motor symptoms of withdrawal, such as escape jumps, were quantified.

Main Results:

Related Experiment Videos

  • Naloxone-induced opiate withdrawal correlated with increased cerebellar NOS activity.
  • L-NAME administration significantly reduced withdrawal symptoms and cerebellar NOS activity.
  • Coadministration of L-arginine with L-NAME reversed these effects.

    • Conclusions:

    • The L-arginine: NO pathway is hyperactive during opiate withdrawal.
    • NOS inhibitors show potential as a therapeutic strategy for managing opiate withdrawal.