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Related Experiment Videos

Selection for genes encoding secreted proteins and receptors

R D Klein1, Q Gu, A Goddard

  • 1Department of Neuroscience, Genentech Inc., South San Francisco, CA 94080-4990, USA.

Proceedings of the National Academy of Sciences of the United States of America
|July 9, 1996
PubMed
Summary

Researchers developed a novel yeast-based method to efficiently identify genes encoding extracellular proteins. This technique successfully isolated 559 candidate genes, advancing the study of cell signaling and disease mechanisms.

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Area of Science:

  • Molecular Biology
  • Proteomics
  • Cell Biology

Background:

  • Extracellular proteins are crucial for multicellular organism development and function.
  • Systematic identification of genes encoding these proteins has been a significant challenge.
  • Understanding extracellular proteins is key to deciphering biological processes and diseases.

Purpose of the Study:

  • To develop and apply a highly efficient method for isolating genes encoding secreted and membrane-bound proteins.
  • To identify novel and known extracellular proteins from various tissues.
  • To facilitate a comprehensive understanding of extracellular signaling molecules.

Main Methods:

  • A single-step selection method in yeast, termed signal peptide selection, was employed.

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  • This technique isolates genes based on the presence of signal peptides, indicative of secreted or membrane proteins.
  • The method was applied to diverse tissue samples.

    • Main Results:

    • The signal peptide selection method yielded 559 candidate clones.
    • These clones represent known and potentially novel extracellular proteins.
    • Identified proteins include growth factors, hormones, receptors, adhesion molecules, and ion channels.

    Conclusions:

    • Signal peptide selection is an effective strategy for identifying extracellular protein-encoding genes.
    • This method significantly advances the potential for comprehensive cataloging of extracellular signaling molecules.
    • Further identification will enhance understanding of fundamental biology and disease intervention strategies.