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Related Experiment Videos

CD45 engagement induces L-selectin down-regulation

D Stibenz1, C Bührer, D Laufer

  • 1Department of Neonatology, Children's Hospital, Virchow Medical Center, Humboldt University, Berlin, Germany.

Scandinavian Journal of Immunology
|July 1, 1996
PubMed
Summary

CD45 glycoprotein regulates L-selectin (CD62L) expression on leukocytes. This study shows CD45

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Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • CD45 glycoprotein isoforms possess intracellular protein tyrosine phosphatase (PTPase) activity.
  • L-selectin (CD62L) is a crucial leukocyte glycoprotein involved in cell adhesion and migration.
  • The precise regulatory mechanisms of L-selectin expression remain incompletely understood.

Purpose of the Study:

  • To investigate the role of CD45 in the regulation of L-selectin (CD62L) surface expression on lymphocytes and granulocytes.
  • To elucidate the involvement of PTPase activity, protein tyrosine kinase (PTK) signaling, and protein kinase C (PKC) in CD45-mediated L-selectin regulation.

Main Methods:

  • Utilized monoclonal antibodies (MoAbs) targeting CD45 epitopes.
  • Employed chemical inhibitors including herbimycin A (PTK inhibitor) and H 7 (PKC inhibitor).
  • Used vanadate as a PTPase inhibitor and phorbol 12-myristate 13-acetate (PMA) for PKC activation.

Main Results:

  • Anti-CD45 MoAbs induced L-selectin down-regulation on lymphocytes, enhanced by cell density and partially inhibited by herbimycin A.
  • Vanadate, a CD45 PTPase inhibitor, induced L-selectin down-regulation on both lymphocytes and granulocytes.
  • PMA and H 7 modulated L-selectin expression, with synergistic or antagonistic effects observed with vanadate depending on cell type.

Conclusions:

  • CD45 plays a significant role in regulating L-selectin surface expression in a cell type-specific manner.
  • Tyrosine phosphorylation/dephosphorylation cascades and the PKC system are key components in the regulation of L-selectin.
  • These findings contribute to understanding leukocyte adhesion and immune cell trafficking.

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