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Related Experiment Videos

Tumor-infiltrating lymphocytes. Cytologic, phenotypic and morphometric analysis

G Oliver1, J Yannelli, D Solomon

  • 1Cytopathology Section, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.

Acta Cytologica
|July 1, 1996
PubMed
Summary

Tumor-infiltrating lymphocytes (TILs) show distinct cytologic and morphometric features compared to normal lymphocytes. These activated TILs, with increased cell size, may explain differences in their trafficking patterns.

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Area of Science:

  • Immunology
  • Oncology
  • Cell Biology

Background:

  • Tumor-infiltrating lymphocytes (TILs) play a crucial role in anti-tumor immunity.
  • Understanding the characteristics of TILs is essential for cancer immunotherapy.
  • Distinct features of TILs may influence their function and localization within the tumor microenvironment.

Purpose of the Study:

  • To characterize the cytologic, phenotypic, and morphometric features of tumor-infiltrating lymphocytes (TILs).
  • To compare these features with normal peripheral blood lymphocytes (PBLs).

Main Methods:

  • Morphological analysis of 50 TIL and 5 normal PBL specimens using light microscopy.
  • Manual ocular measurements and digital image processing for cell size and parameter assessment.

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Main Results:

  • TILs exhibited consistent cytologic changes across different tumor types, including large, eccentric nuclei, clumped chromatin, and prominent nucleoli.
  • Abundant, basophilic cytoplasm and evident mitotic figures were observed in TILs.
  • Significant increases in cell size parameters were noted in TILs compared to PBLs.

Conclusions:

  • The activated morphology of TILs resembles that of high-grade lymphoma.
  • Increased cell size parameters in TILs may contribute to observed differences in trafficking patterns compared to PBLs.
  • These findings enhance our understanding of TIL biology and potential implications for cancer treatment.