[Effect of vitamin E and vitamin C deficiency on the development of alcoholic liver diseases]

Effects of vitamin E (V.E) and vitamin C (V.C) deficiency on the progress of alcoholic liver disorder in 72 Osteogenic Disorder Shionogi (ODS) male rats were studied. Animals were divided into two groups which received 5% ethanol liquid diet (A group) or pair-fed an isocalorical liquid diet with carbohydrate replacing ethanol (C group). All animals were pair-fed with these diets free of V.E during experiment period. In first experiment, after pair-feeding with the diets free of V.C for 2 or 5 weeks, the animals were killed. In another experiment, after pair-feeding with the diets free of V.C for 4 weeks, and then with the diets addition of 0.1 or 1.0 mg/ml V.C. for 2 weeks, the animals were killed. In first experiment, hepatic V.E concentrations in rats fed with V.E. and V.C deficient diets decreased more severely than those fed with only V.E deficient diets. After 2 weeks hepatic lipid peroxide content was increased, while at 5 weeks, it's decreased both in A and C group. In another experiment, hepatic aniline hydroxylase activity increased both by ethanol administration and by addition of V.C. Total P450 and P450 2E1 contents also increased by addition of V.C. And then hepatic lipid peroxide contents increased by addition of V.C. in A groups. These results suggested that V.E and V.C deficiency disturbed alcohol-metabolic enzyme activity on the progress of alcoholic liver disorder.