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Related Experiment Videos

Membrane receptor for odour-binding proteins

M Boudjelal1, A Sivaprasadarao, J B Findlay

  • 1Department of Biochemistry and Molecular Biology, University of Leeds. U.K.

The Biochemical Journal
|July 1, 1996
PubMed
Summary
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Researchers identified a specific receptor for bovine odour-binding protein (OBP) in nasal membranes. This OBP receptor, found in various tissues, suggests broader roles beyond olfaction, potentially in detoxification or signaling.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Neuroscience

Background:

  • Odour-binding proteins (OBPs) are implicated in olfaction.
  • The specific receptors and broader functions of OBPs are not fully understood.

Purpose of the Study:

  • To characterize the binding of bovine OBP to nasal mucosa membranes.
  • To investigate the nature and distribution of the OBP receptor.

Main Methods:

  • Used 125I-labelled bovine OBP to study binding kinetics and Scatchard analysis.
  • Investigated receptor activity using enzymatic digestion, heat treatment, and thiol-specific reagents.
  • Tested cross-reactivity with other lipocalin family members.

Main Results:

  • Demonstrated specific, reversible binding of bovine OBP to nasal membranes.

Related Experiment Videos

  • Scatchard analysis indicated a single binding site population (Kd = 2.25 µM, Bmax = 18.5 pmol/mg).
  • Receptor activity was sensitive to proteases, heat, and thiol reagents, but not other lipocalins except for rat and mouse major urinary proteins.

    • Conclusions:

    • A specific OBP receptor exists in nasal mucosa with defined binding characteristics.
    • The receptor's presence in non-olfactory tissues suggests functions beyond olfaction, possibly in detoxification or signaling.