Related Experiment Videos
Mechanisms and uses of hydrogen exchange
S W Englander1, T R Sosnick, J J Englander
1Johnson Research Foundation, Department of Biochemistry & Biophysics, University of Pennsylvania School of Medicine, Philadelphia 19104-6059, USA. walter@hx2.med.upenn.edu
Current Opinion in Structural Biology
|February 1, 1996
Abstract:
Recent work has largely completed our understanding of the hydrogen-exchange chemistry of unstructured proteins and nucleic acids. Some of the high-energy structural fluctuations that determine the hydrogen-exchange behavior of native macromolecules have been explained; others remain elusive. A growing number of applications are exploiting hydrogen-exchange behavior to study difficult molecular systems and elicit otherwise inaccessible information on protein structure, dynamics and energetics.