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The TATA box binding protein

S K Burley1

  • 1Laboratories of Molecular Biophysics, Rockefeller University, New York, NY 10021, USA.

Current Opinion in Structural Biology
|February 1, 1996
PubMed
Summary
This summary is machine-generated.

The TATA box binding protein (TBP) is crucial for initiating transcription by all eukaryotic RNA polymerases. Recent structural studies reveal TBP alone, bound to TATA elements, and in a complex with transcription factor IIB.

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Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • The TATA box binding protein (TBP) is a fundamental transcription factor essential for gene expression in eukaryotes.
  • TBP interacts with the TATA element, a conserved DNA sequence, to initiate transcription by RNA polymerases I, II, and III.
  • Extensive biochemical and genetic research has been conducted on TBP since its initial cloning from Saccharomyces cerevisiae.

Purpose of the Study:

  • To elucidate the structural and mechanistic aspects of TATA box binding protein (TBP).
  • To provide insights into the molecular interactions governing transcription initiation.

Main Methods:

  • X-ray crystallography was employed to determine the three-dimensional structures.
  • Studies focused on TBP alone, TBP bound to TATA elements, and ternary complexes.

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Main Results:

  • Newly determined structures include TBP in isolation and TBP bound to various TATA elements.
  • The structure of a ternary complex involving TBP, a TATA element, and transcription factor IIB was elucidated.
  • These structures provide detailed atomic-level information on TBP-DNA and TBP-protein interactions.

Conclusions:

  • Recent structural advancements offer a deeper understanding of TBP's role in transcription initiation.
  • The elucidated structures facilitate mechanistic studies of eukaryotic gene regulation.
  • These findings contribute to the broader field of molecular biology and gene expression research.