Stimulation of arachidonic acid mobilization by adherence of resident peritoneal macrophages to plastic substrate

To interpret results of studies on arachidonic acid (AA) mobilization and metabolism in vitro, it is essential that the influence of culture and conditions should be well defined. Thus, we investigated the effects of murine resident peritoneal macrophage adherence and the presence of foetal calf serum in culture medium on arachidonic acid mobilization. The present data demonstrate that [3H] AA mobilization was triggered simply by contact between cell and substrate. The presence of serum can modulate cell-substrate interactions but not AA mobilization. Protein kinase C, and calmodulin inhibitors failed to inhibit [3H] AA release induced by cell adherence. Finally, low molecular weight PLA2 inhibitors were not able to inhibit [3H] AA mobilization stimulated by cell adherence.