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[Biotherapy of advanced cancer by infusion of in vitro activated autolymphocytes]
1Department of Microbiology and Immunology, Henan Medical University, Zhengzhou.
Autolymphocyte biotherapy is an adoptive cellular therapy based upon the infusion of autologous lymphocytes that have been activated in vitro by anti-CD3 monoclonal antibody and depleted of suppressor T cells by cimetidine, indomethacin and low dose of gamma irradiation. The patients also receive oral cimetidine to block suppressor T cell activity in vivo. Sixty five patients received 217 autolymphocyte infusions and toxicity was minimal with transient fever and chill accompanying 18 infusions. The efficacy of treatment in patients received more than 3 infusions were evaluated. In 37 evaluable patients, the response rate (CR+PR) was 24% (9/37). As the side reactions are mild, the treatment can be done on an outpatient basis.
Autolymphocyte biotherapy is an adoptive cellular therapy based upon the infusion of autologous lymphocytes that have been activated in vitro by anti-CD3 monoclonal antibody and depleted of suppressor T cells by cimetidine, indomethacin and low dose of gamma irradiation. The patients also receive oral cimetidine to block suppressor T cell activity in vivo. Sixty five patients received 217 autolymphocyte infusions and toxicity was minimal with transient fever and chill accompanying 18 infusions. The efficacy of treatment in patients received more than 3 infusions were evaluated. In 37 evaluable patients, the response rate (CR+PR) was 24% (9/37). As the side reactions are mild, the treatment can be done on an outpatient basis.