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Related Experiment Videos

CD20 (pan-B cell antigen) expression on bone marrow-derived T cells

K M Algino1, R W Thomason, D E King

  • 1Department of Pathology, University of Texas Health Science Center, San Antonio 78284, USA.

American Journal of Clinical Pathology
|July 1, 1996
PubMed
Summary

CD20 antibodies, typically used for B lymphocytes, also identify CD20-positive T cells in bone marrow. This finding is common in normal and abnormal samples, requiring caution when interpreting CD20 expression in T-cell lymphomas.

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Area of Science:

  • Immunology
  • Hematology
  • Oncology

Background:

  • Antibodies targeting CD20 are standard for identifying B lymphocytes.
  • Recent studies reveal CD20 expression on T cells in peripheral blood and some T-cell cancers.
  • The presence and significance of CD20-positive T cells in bone marrow remain less understood.

Purpose of the Study:

  • To investigate the prevalence and characteristics of CD20-positive T cells within bone marrow aspirate specimens.
  • To assess potential correlations between CD20-positive T cells and clinical parameters or diagnoses.
  • To clarify the implications of CD20 expression on T-lymphoid cells in bone marrow analysis.

Main Methods:

  • Flow cytometry was employed to analyze 34 bone marrow aspirate specimens.

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  • Specimens included normal controls and various hematologic malignancies (ALL, AML, CML, CLL, multiple myeloma) and HIV-positive cases.
  • Cells were analyzed for coexpression of CD3 (T-cell marker) and CD20 (B-cell marker).

    • Main Results:

    • A CD3+/CD20dim population was detected in 94% of bone marrow specimens.
    • This population represented a small percentage of total mononuclear cells (mean 1.77%) and lymphoid cells (mean 6.54%).
    • No significant correlation was found between CD20-positive T cells and CD4:CD8 ratio, age, gender, or specific diagnosis.

    Conclusions:

    • CD20-positive T cells are frequently present in bone marrow, including in normal individuals.
    • The CD20dim population includes both immature B cells and T cells.
    • Interpretation of CD20 expression in bone marrow T-lymphoid cells requires caution, as it can occur in normal, reactive, or neoplastic conditions.