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Related Experiment Videos

Simple procedure for measuring total protein in urine

J M Meola, M A Vargas, H H Brown

    Clinical Chemistry
    |June 1, 1977
    PubMed
    Summary

    This study introduces a novel, sensitive method for measuring total urine protein. The procedure is reliable for routine use and avoids interference from common drugs, offering accurate results.

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    Area of Science:

    • Clinical Chemistry
    • Biochemical Assays

    Background:

    • Accurate measurement of total urine protein is crucial for diagnosing and monitoring kidney disease.
    • Existing methods for urine protein quantification can be affected by drug interference and may lack sensitivity.

    Purpose of the Study:

    • To develop and validate a simple, sensitive, and drug-interference-free method for measuring total protein in urine.

    Main Methods:

    • Protein adsorption onto cellulose powder.
    • Ponceau S dye binding to urinary proteins.
    • Washing to remove unbound dye.
    • Elution of bound dye in dilute NaOH for colorimetric analysis.

    Main Results:

    • The developed method demonstrates high sensitivity and is free from interference by common drugs.
    • The coefficient of variation (CV) for a 1.1 g/liter urine control was 4.6% in daily, routine use.
    • Good agreement was observed when comparing results with biuret and turbidimetric methods for suitable specimens.

    Conclusions:

    • This new procedure offers a reliable and accurate alternative for routine total urine protein measurement.
    • Its simplicity and resistance to drug interference make it a valuable tool in clinical diagnostics.

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