Related Experiment Video
Updated: Aug 3, 2026

Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes
Published on: October 23, 2014
Nucleosome disruption by human SWI/SNF is maintained in the absence of continued ATP hydrolysis
A N Imbalzano1, G R Schnitzler, R E Kingston
1Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA.
Abstract:
We have examined the requirement for ATP in human (h) SWI/SNF-mediated alteration of nucleosome structure and facilitation of transcription factor binding to nucleosomal DNA. hSWI/SNF-mediated nucleosome alteration requires hydrolysis of ATP or dATP. The alteration is stable upon removal of ATP from the reaction or upon inhibition of activity by excess ATPgammaS, indicating that continued ATP hydrolysis is not required to maintain the altered nucleosome structure. This stable alteration is sufficient to facilitate binding of a transcriptional activator protein; concurrent ATP hydrolysis was not required to facilitate binding. These data suggest sequential steps that can occur in the process by which transcription factors gain access to nucleosomal DNA.
Related Concept Videos
Nucleosome Remodeling
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
DNA Damage can Stall the Cell Cycle
Restarting Stalled Replication Forks
ATP Synthase: Mechanism
Destabilization of Microtubules
DNA Damage Can Stall the Cell Cycle

