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Neutrophil transepithelial migration is dependent upon epithelial characteristics
1University of Iowa College of Medicine, Iowa City, USA.
American Journal of Respiratory Cell and Molecular Biology
|August 1, 1996
Summary
Neutrophil migration to airways during inflammation depends on epithelial cell type. Lung epithelial cells produce interleukin-8 (IL-8), influencing neutrophil transepithelial migration in response to inflammatory signals.
Area of Science:
- Immunology
- Cell Biology
- Respiratory Medicine
Background:
- Neutrophil migration is crucial for inflammatory responses in the airways.
- Understanding epithelial cell involvement in neutrophil recruitment is key to managing airway inflammation.
Purpose of the Study:
- To investigate the mechanisms of neutrophil transepithelial migration across lung epithelial cells.
- To compare neutrophil migration in response to various chemoattractants and cytokines across different epithelial cell lines.
Main Methods:
- An in vitro model using three human lung epithelial cell lines (A549, H441, 16-HBE-14o).
- Exposure of epithelial monolayers to chemoattractants (FMLP, LTB4, IL-8) and cytokines (IL-1α, IL-1β, TNFα).
- Measurement of neutrophil migration across epithelial layers and assessment of epithelial IL-8 production.
Main Results:
- Neutrophil migration was induced by FMLP, LTB4, and IL-8 across all tested epithelia.
- TNFα- and IL-1-induced migration occurred only in epithelia that produced soluble chemoattractants in response to these cytokines.
- Epithelial IL-8 production capacity correlated with the ability to induce transepithelial migration upon stimulation with IL-1 and TNFα.
Conclusions:
- Epithelial cell phenotype, specifically IL-8 production capacity, significantly influences stimulated neutrophil transepithelial migration.
- Lung epithelial cells play an active role in regulating neutrophil recruitment to the airways during inflammation.