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Related Experiment Videos

Are there functional gap junctions or junctional hemichannels in macrophages?

L A Alves1, R Coutinho-Silva, P M Persechini

  • 1Departamento de Imunologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.

Blood
|July 1, 1996
PubMed
Summary

Macrophages do not form functional gap junctions, even when stimulated. Extracellular ATP-activated permeability is not mediated by hemichannel channels, indicating immune cell communication differs from other cell types.

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Area of Science:

  • Immunology
  • Cell Biology
  • Biophysics

Background:

  • The presence of functional gap junctions in immune cells, particularly macrophages, is debated.
  • Connexin-43 (Cx43) has been detected in macrophages, suggesting potential gap junction formation.

Purpose of the Study:

  • To investigate whether macrophages form functional gap junctions and explore ATP-activated permeability pathways.

Main Methods:

  • Dye coupling assays (Lucifer Yellow microinjection)
  • Dual whole-cell patch-clamp recordings
  • ATP-induced dye uptake assays
  • Experiments with connexin-43 knockout mice

Main Results:

  • Nonstimulated and activated macrophages showed no dye or electrical coupling with themselves or epithelial cells.

Related Experiment Videos

  • Extracellular ATP-induced permeability in macrophages was not mediated by hemichannel channels.
  • ATP-induced dye uptake occurred in macrophages from Cx43 knockout mice.

    • Conclusions:

    • Macrophages in vitro do not form functional gap junctions.
    • The permeability pathway activated by extracellular ATP in macrophages is not a hemigap junction channel.