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Related Experiment Videos

Experimental Bacteroides fragilis endocarditis in rabbits

J Carrizosa, D Tanphaichitra, M E Levison

    Infection and Immunity
    |March 1, 1977
    PubMed
    Summary

    This study demonstrates that a specific strain of Bacteroides fragilis causes endocarditis in rabbits, with left-sided catheters requiring a lower infective dose. This experimental model is valuable for testing treatments for Bacteroides fragilis endocarditis.

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    Area of Science:

    • Microbiology
    • Infectious Diseases
    • Cardiovascular Research

    Background:

    • Bacteroides fragilis is an opportunistic pathogen capable of causing serious infections, including endocarditis.
    • Understanding the pathogenesis of B. fragilis endocarditis is crucial for developing effective treatments.
    • Previous studies have lacked a robust experimental model to investigate this condition.

    Purpose of the Study:

    • To characterize Bacteroides fragilis endocarditis using a novel rabbit experimental model.
    • To evaluate the impact of catheter placement (left-sided vs. right-sided) on infective dose.
    • To establish a reproducible model for testing therapeutic interventions.

    Main Methods:

    • Utilized a serum-resistant, non-heparinase-producing strain of Bacteroides fragilis.
    • Inoculated rabbits with varying doses of B. fragilis, with and without intracardiac catheters.
    • Monitored bacterial titers and vegetation weights in different cardiac locations over time.

    Main Results:

    • Significantly lower infective dose (log10 6.3 CFU/ml) required for endocarditis in rabbits with left-sided catheters compared to right-sided (log10 7.7 CFU/ml).
    • Bacterial titers in tricuspid vegetations were lower than aortic vegetations at 3 days post-infection, but similar by day 5.
    • Vegetation weights showed no significant difference between tricuspid and aortic locations at 3 days.
    • No spontaneous deaths occurred, and catheter removal prior to infection did not alter bacterial titers.

    Conclusions:

    • A reproducible rabbit model for studying Bacteroides fragilis endocarditis has been established.
    • Left-sided intracardiac catheters increase susceptibility to B. fragilis endocarditis.
    • This model facilitates the evaluation of antimicrobial drug regimens for treating B. fragilis endocarditis.

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