Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Small nuclear RNA localization during mitosis. An electron microscope study

L Goldstein, G E Wise, C Ko

    The Journal of Cell Biology
    |May 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Stool and urine trefoil factor 3 levels: associations with symptoms, intestinal permeability, and microbial diversity in irritable bowel syndrome.

    Beneficial microbes·2018
    Same author

    Hidden Magnetic States Emergent Under Electric Field, In A Room Temperature Composite Magnetoelectric Multiferroic.

    Scientific reports·2017
    Same author

    A Transplant-Specific Quality Initiative-Introducing TransQIP: A Joint Effort of the ASTS and ACS.

    American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2017
    Same author

    Selective deletion of Pten in theca-interstitial cells leads to androgen excess and ovarian dysfunction in mice.

    Molecular and cellular endocrinology·2017
    Same author

    Twinstar/cofilin is required for regulation of epithelial integrity and tissue growth in Drosophila.

    Oncogene·2016
    Same author

    Hepatitis B virus X protein enhances Myc stability by inhibiting SCF(Skp2) ubiquitin E3 ligase-mediated Myc ubiquitination and contributes to oncogenesis.

    Oncogene·2015

    Small nuclear ribonucleic acids (snRNAs) concentrate in chromosomes during early mitosis but not at metaphase in Amoeba proteus. These findings suggest snRNAs may play a role in chromosome organization.

    Area of Science:

    • Cell Biology
    • Molecular Biology
    • Genetics

    Background:

    • Small nuclear ribonucleic acids (snRNAs) are essential components of the spliceosome.
    • Their dynamic localization during the cell cycle is crucial for gene expression regulation.

    Purpose of the Study:

    • To investigate the precise localization of snRNAs during mitosis in the single-celled organism Amoeba proteus.
    • To understand the dynamic association of snRNAs with chromosomes throughout different mitotic phases.

    Main Methods:

    • High-voltage electron microscope autoradiography utilized to track [3H]uridine-labeled snRNAs.
    • Micromanipulation techniques employed to isolate labeled snRNAs within the cell.

    Main Results:

    • During interphase, snRNAs are primarily nuclear, with higher concentrations over chromatin.

    Related Experiment Videos

  • In prophase, snRNAs strongly associate with condensing chromosomes.
  • At metaphase, snRNAs become predominantly cytoplasmic, detaching from maximally condensed chromosomes.
  • During anaphase and telophase, snRNAs re-associate with chromosomes and return to the nucleus.

    • Conclusions:

    • snRNAs exhibit a differential affinity for chromosomes based on their condensation state.
    • This dynamic localization suggests a potential role for snRNAs in genetic reprogramming or chromosome organization during mitosis.