[The cloning and expression of human secreted interleukin 2 receptor alpha chain gene in eukaryotic cells]

By deleting the sequences encoding the cytoplasmic domain, transmembrane domain and the 5' untranslated region, human interleukin-2 receptor alpha cDNA fragment was subcloned into an eukaryotic expression vector pRc/CMV. The recombinant plasmid was then transfected into CHO cells by either electroporation or calcium phosphate precipitation. 11 G418-resistent clones were randomly selected for detection of mRNA expression. 5 clones out of the 11 were proved to be positively hybridyzed with IL-2R alpha cDNA probe. All of the 5 positive cell lines also expressed sIL-2R alpha in their supernatants as detected by ELISA. The establishment of rhsIL-2R alpha cell lines is of importance in the detection and purification of IL-2 based on the ability of affinity binding between IL-2 and its recombinant receptor.