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Related Experiment Videos

Gluconeogenesis from ascorbic acid: ascorbate recycling in isolated murine hepatocytes

L Braun1, F Puskás, M Csala

  • 1Department of Medical Chemistry, Semmelweis University of Medicine, Budapest, Hungary.

FEBS Letters
|July 22, 1996
PubMed
Summary

This study reveals a novel pathway for ascorbate recycling in mouse liver cells. Ascorbate breakdown and synthesis are linked to glucose production via the pentose phosphate and gluconeogenesis pathways.

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Metabolic Pathways

Background:

  • Ascorbic acid (vitamin C) plays crucial roles in cellular metabolism.
  • Understanding the regulation of its synthesis and breakdown is vital for metabolic research.
  • Previous studies have not fully elucidated the interplay between ascorbate metabolism and glucose production.

Purpose of the Study:

  • To investigate the synthesis and breakdown of ascorbic acid in isolated mouse hepatocytes.
  • To explore the relationship between ascorbate metabolism and glucose production.
  • To identify novel pathways involved in ascorbate recycling.

Main Methods:

  • Isolated hepatocytes from fasted mice were used.
  • Stimulation of gluconeogenesis using alanine or xylitol.

Related Experiment Videos

  • Addition of ascorbate or dehydroascorbate to cell cultures.
  • Measurement of glucose production and pentose phosphate pathway intermediates.
  • Inhibition of specific metabolic pathways.

    • Main Results:

    • Gluconeogenesis stimulation led to ascorbate synthesis.
    • Ascorbate or dehydroascorbate addition increased glucose production and xylulose 5-phosphate levels.
    • Stimulating ascorbate oxidation or inhibiting dehydroascorbate reduction enhanced glucose formation.
    • Pentose phosphate pathway inhibition reduced dehydroascorbate-driven glucose production and increased xylulose 5-phosphate accumulation.

    Conclusions:

    • Ascorbate can be recycled through a novel pathway.
    • This pathway involves intermediates from the pentose phosphate pathway, gluconeogenesis, and the hexuronic acid pathway.
    • The findings provide new insights into the metabolic regulation of ascorbate and glucose homeostasis.