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Characterisation of invasive Haemophilus influenzae isolated in Nagpur, central India
V Agarwal1, D Jain, A A Pathak
1Department of Microbiology, Government Medical College & Hospital, Nagpur.
Abstract:
A total of 14 invasive H. influenzae were isolated from 12 patients by culturing 621 samples of body fluids over a span of one year. Twelve non-replicate isolates were analyzed for their drug susceptibility and serotype distribution. Eight (66.7%) isolates were multiply drug resistant with resistance to ampicillin, chloramphenicol and cotrimoxazole in different combinations. All isolates were susceptible to cefotaxime and erythromycin. Serotype b accounted for 10 (83.3%) isolates while one each were type a and non-typeable respectively. A continuous surveillance of drug susceptibility and serotype distribution is important to determine changes in the behaviour of the organism over time.
Insights
Invasive Haemophilus influenzae (H. influenzae) infections show high resistance to common antibiotics like ampicillin. Continuous monitoring of drug resistance and serotypes is crucial for effective treatment strategies.
Area of Science:
- Medical Microbiology
- Infectious Diseases
- Bacteriology
Background:
- Invasive *Haemophilus influenzae* infections pose a significant public health challenge.
- Understanding antimicrobial resistance patterns and serotype distribution is vital for managing infections.
Purpose of the Study:
- To analyze the drug susceptibility and serotype distribution of invasive *H. influenzae* isolates.
- To identify trends in antimicrobial resistance and prevalent serotypes.
Main Methods:
- Culturing of body fluid samples to isolate *H. influenzae*.
- Antimicrobial susceptibility testing (AST) for various drugs.
- Serotyping of isolates to determine type distribution.
Main Results:
- Eight out of twelve (66.7%) *H. influenzae* isolates exhibited multidrug resistance (MDR).
- Common resistance observed against ampicillin, chloramphenicol, and cotrimoxazole.
- All isolates remained susceptible to cefotaxime and erythromycin.
- Serotype b was the predominant type (83.3%), with one isolate each of type a and non-typeable.
Conclusions:
- High rates of MDR *H. influenzae* necessitate careful antibiotic selection.
- Serotype b is a major cause of invasive infections.
- Ongoing surveillance of drug resistance and serotypes is essential for public health interventions.