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Related Experiment Videos

Blood-pool imaging using technetium-99m-labeled liposomes

B Goins1, W T Phillips, R Klipper

  • 1Department of Radiology, University of Texas Health Science Center at San Antonio, Texas 78284-7800, USA.

Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine
|August 1, 1996
PubMed
Summary
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Technetium-99m-liposomes demonstrate superior circulation persistence and stability for blood-pool imaging compared to standard agents. Polyethylene glycol (PEG) modified liposomes offer advantages over neutral liposomes and red blood cells.

Area of Science:

  • Nuclear Medicine
  • Radiopharmacy
  • Biomedical Imaging

Background:

  • Blood-pool imaging agents are crucial for various diagnostic procedures.
  • Standard agents like 99mTc-red cells and 99mTc-human serum albumin (HSA) have limitations.
  • Development of novel, stable blood-pool agents is an ongoing area of research.

Purpose of the Study:

  • To evaluate two 99mTc-labeled liposome formulations as potential blood-pool imaging agents.
  • To compare their performance against standard 99mTc-red cells and 99mTc-HSA.
  • To assess the impact of surface modification (PEGylation) on liposome behavior.

Main Methods:

  • Two liposome formulations (unmodified and PEG-coated) were labeled with 99mTc using HMPAO.
  • Autologous red blood cells and commercial 99mTc-HSA served as controls.

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  • In vivo studies in rabbits involved intravenous injection and image acquisition.

    • Main Results:

    • 99mTc-liposomes exhibited prominent blood-pool activity with lower bladder burden than controls.
    • PEGylated 99mTc-liposomes showed reduced liver activity compared to neutral liposomes.
    • PEGylated 99mTc-liposomes demonstrated 1.6 times longer circulation persistence.

    Conclusions:

    • 99mTc-liposomes offer excellent circulation persistence and in vivo stability.
    • PEGylated 99mTc-liposomes provide superior performance due to reduced liver background.
    • PEGylated 99mTc-liposomes present several advantages over 99mTc-red cells for blood-pool imaging.