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Related Experiment Videos

Transcription antitermination: the lambda paradigm updated

D I Friedman1, D L Court

  • 1Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109-620, USA. davidfri@umich.edu

Molecular Microbiology
|October 1, 1995
PubMed
Summary
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Bacteriophage lambda uses transcription antitermination to control gene expression. Recent studies reveal N protein

Area of Science:

  • Molecular Biology
  • Microbiology
  • Virology

Background:

  • Bacteriophage lambda regulates gene expression through transcription termination and antitermination.
  • The N protein and host Nus factors control early gene expression by modifying RNA polymerase.
  • The Q protein regulates late gene expression via a similar mechanism.

Purpose of the Study:

  • To review recent findings on transcription antitermination mechanisms in bacteriophage lambda and related phages.
  • To elucidate the roles of phage and host factors in modulating RNA polymerase activity.
  • To investigate the signals and interactions governing termination resistance.

Main Methods:

  • In vitro transcription assays to assess N-mediated antitermination.
  • Analysis of antitermination in the lambdoid phage HK022.

Related Experiment Videos

  • Investigation of DNA-templated signals for Q-mediated antitermination.
  • Biochemical studies on the interaction of antitermination factors with RNA polymerase and inhibitors.

    • Main Results:

    • N protein can mediate antitermination in vitro independently of Nus proteins.
    • Antitermination of early genes in phage HK022 requires only an RNA signal.
    • A portion of the Q utilization site (qut) signal is derived from the non-template DNA strand.
    • A host inhibitor of N antitermination targets the NUT site and RNA polymerase alpha subunit, and is opposed by NusB.

    Conclusions:

    • Transcription antitermination is a versatile regulatory mechanism in bacteriophages.
    • Distinct phage proteins (N and Q) employ specific signals and host factors to achieve termination resistance.
    • Understanding these mechanisms provides insights into gene regulation and phage-host interactions.