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Related Experiment Videos

CD117/CD34 expression in leukemic blasts

S J Wells1, R A Bray, L L Stempora

  • 1Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia, USA.

American Journal of Clinical Pathology
|August 1, 1996
PubMed
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CD117 is frequently expressed on myeloid leukemia blasts, including acute myelogenous leukemia (AML) and myelodysplastic syndromes (MDS). Its expression does not correlate with CD34 positivity or lymphoid-associated antigen expression in AML.

Area of Science:

  • Hematology
  • Oncology
  • Immunophenotyping

Background:

  • CD117, a receptor for stem cell factor, is expressed on myeloid cells and certain leukemias.
  • CD34 is a primitive stem cell marker often associated with poor prognosis in leukemia.
  • The relationship between CD117 and CD34 expression, and lymphoid markers in leukemia, requires further investigation.

Purpose of the Study:

  • To investigate the correlation between CD117 and CD34 expression on leukemic blasts.
  • To determine if CD117 expression relates to lymphoid-associated antigen (LAA) expression in acute myelogenous leukemia (AML).

Main Methods:

  • Multiparameter flow cytometry was used to analyze CD117 and CD34 expression on bone marrow samples.
  • Samples included cases of AML, myelodysplastic syndromes (MDS), myeloproliferative disorders in blast crisis (MPD-BC), and acute lymphoblastic leukemia (ALL).

Related Experiment Videos

  • Expression of lymphoid-associated antigens (CD4, CD7, CD2) was assessed in CD117+ AML cases.

    • Main Results:

    • CD117 was expressed on a high percentage of myeloid leukemia blasts (87% AML, 80% MPD-BC, 75% MDS).
    • CD117/CD34 expression patterns varied across different leukemia subtypes and disease phases (de novo vs. relapsed).
    • CD117 expression on AML blasts did not exclude the expression of lymphoid-associated antigens.

    Conclusions:

    • CD117 is a common marker on myeloid leukemia blasts and its expression is largely independent of CD34 status.
    • CD117 expression does not preclude the presence of lymphoid markers on AML blasts.
    • CD117 expression may serve as a myeloid marker in leukemia, irrespective of CD34 expression.