Postnatal development of ovine seminiferous tubules: an electron microscopical and morphometric study

Corresponding to the increasing testicular volume and the histological appearance of the testicular parenchyma, the postnatal ontogenesis of the ovine testis can be divided into five phases. During the prebubertal period (phases 1-III), seminiferous tubules are solid and contain supporting (pre-Sertoli) cells as well as up to three types of germ cells: prespermatogonia I, II and spermatogonia precursor cells. In phase I, only prespermatogonia I are present and can usually be observed at the center of the seminiferous tubules. During phase II, prespermatogonia I migrate towards the basal lamina, divide and become prespermatogonia II. Those prespermatogonia I which are not successful in establishing contact with the tubular basal lamina degenerate. In phase III, prespermatogonia II divide and differentiate into cells which function as stem cells for spermatogenesis. Morphometric data corroborate the assumption of two types of prespermatogonia in the postnatal prepubertal ovine testis. Prespermatogonia I have nuclear volumes of about 480 microns 3 and cellular volumes of about 1200 microns3. In prespermatogonia II both volumes increase to about 920 microns3 and 1800 microns3 respectively. Adult A-spermatogonia are significantly smaller and possess an average nuclear volume of about 340 microns3 and an average cellular volume of about 800 microns3. Concomitanty with the formation of the tubular lumen in puberty (phase IV), supporting cells differentiate morphologically into typical Sertoli cells. Developmental events in the germ cell population are not yet synchronized. Adulthood (phase V) is characterized by complete spermatogenesis with all stages of the seminiferous epithelial cycle.