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Pseudosubstrate flexibility in chicken smooth muscle myosin light chain kinase
1Institute for Biomedical Research, University of Sydney, New South Wales, Australia.
Biochemical and Biophysical Research Communications
|July 25, 1996
Abstract:
Chicken smooth muscle myosin light chain kinase pseudosubstrate is contained within the C-terminal region of the sequence MLCK(774-807)amide. It forms a helical conformation which interacts with the connecting peptide contained within the N-terminal portion of the sequence. These regions are linked by a well-defined turn around Ser-787 which supports a model of pseudosubstrate regulation involving the hinging of the pseudosubstrate or autoregulatory recognition sequence away from the active site of the catalytic core following the binding of calmodulin to the kinase.