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Polymerase chain reaction-based site-directed mutagenesis using magnetic beads
J Fieschi1, P Niccoli, C Camilla
1Laboratoire d'Ingénierie et Dynamique des Systèmes Membranaires, Institut de Biologie Structurale et de Microbiologie, CNRS, Marseille, France.
Analytical Biochemistry
|February 15, 1996
Abstract:
Thyroglobulin double mutants with various substitutions were obtained with a new polymerase chain reaction-based mutagenesis technique. Maximum length of megaprimer and efficiency of mutagenesis were improved by purification of single-stranded DNA, using the avidin-biotin interaction. This method might allow the construction of large libraries of DNA, mutated at different sites.