Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cell death in the embryonic developing limb

J M Hurle1, M A Ros, V Garcia-Martinez

  • 1Departamento de Anatomía y Biología Celular, Universidad de Cantabria, Santander, Spain.

Scanning Microscopy
|June 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Traffic and functional polarization of macrophages in the areas of programmed interdigital cell death in the embryonic chick.

Apoptosis : an international journal on programmed cell death·2026
Same author

Potential risk of macro-plastic on the megafauna of two semi-enclosed European seas.

Marine pollution bulletin·2025
Same author

Multiple trauma management in mountain environments - a scoping review : Evidence based guidelines of the International Commission for Mountain Emergency Medicine (ICAR MedCom). Intended for physicians and other advanced life support personnel.

Scandinavian journal of trauma, resuscitation and emergency medicine·2020
Same author

Pharmacotherapy failure and progression to botulinum toxin injection in vestibular migraine.

The Journal of laryngology and otology·2020
Same author

Identifying distribution and accumulation patterns of floating marine debris in the Black Sea.

Marine pollution bulletin·2020
Same author

Predictors of mortality and neurological function in ICU patients recovering from cardiac arrest: A Spanish nationwide prospective cohort study.

Medicina intensiva·2020
Same journal

Deposition of supercoiled DNA on mica for scanning force microscopy imaging.

Scanning microscopy·1996
Same journal

Relative intranuclear magnesium and phosphorus contents in normal and tumor cells of the human thyroid gland as revealed by energy-dispersive X-ray microanalysis.

Scanning microscopy·1996
Same journal

Preparation of cultured smooth muscle cells from human myometrium for X-ray microanalysis.

Scanning microscopy·1996
Same journal

Combined bronchoalveolar-vascular casting of the canine lung.

Scanning microscopy·1996
Same journal

Effects of castration upon the morphology of the accessory sex organs of the male rat--a scanning electron microscopy study.

Scanning microscopy·1996
Same journal

Scanning electron microscopy of the accessory sex glands of the adult male rat.

Scanning microscopy·1996
See all related articles

Programmed cell death, or apoptosis, is crucial for limb development in vertebrates. Understanding the genetic control of this process is essential for preventing limb malformations.

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Genetics

Background:

  • Limb bud development in amniotes involves significant mesodermal cell death, resembling apoptosis.
  • This programmed cell death eliminates transient, undifferentiated cells essential for specific developmental stages.
  • Altered cell death patterns in limb development lead to predictable skeletal and morphological anomalies.

Purpose of the Study:

  • To investigate the mechanisms triggering cell death during limb development.
  • To explore the genetic underpinnings of cell death in the developing limb.
  • To clarify the role of cell death genes, specifically bcl-2, in limb morphogenesis.

Main Methods:

  • Analysis of cell death patterns in normal and mutant vertebrate limb development.

Related Experiment Videos

  • Experimental manipulation of limb development to observe effects on cell death.
  • Review of existing literature on cell death genes (e.g., p-53, ced-3/ICE, ced-9/bcl-2) in developmental contexts.

    • Main Results:

    • Cell death in limb development is likely initiated by local triggers, possibly involving extracellular matrix modifications or growth factor signaling.
    • Existing data on the bcl-2 gene's role in limb development is contradictory; it's not expressed in cell death areas, yet its disruption doesn't prevent normal limb formation in mice.
    • Information regarding the genetic basis of cell death in developing limbs remains scarce.

    Conclusions:

    • Local environmental cues, such as extracellular matrix and growth factors, are probable triggers for apoptosis during limb development.
    • The precise function of cell death genes, including bcl-2, in vertebrate limb morphogenesis requires further investigation.
    • Future research must focus on elucidating the genetic control of cell death for a comprehensive understanding of limb development and malformations.