Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Altered brain sodium channel transcript levels in human epilepsy

A J Lombardo1, R Kuzniecky, R E Powers

  • 1Department of Psychiatry and Behavioral Neurobiology, University of Alabama at Birmingham 35294-0017, USA.

Brain Research. Molecular Brain Research
|January 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Urinothorax: an under-reported cause of pleural effusions.

QJM : monthly journal of the Association of Physicians·2022
Same author

Corpus callosum area and brain volume in autism spectrum disorder: quantitative analysis of structural MRI from the ABIDE database.

Journal of autism and developmental disorders·2015
Same author

Abstracts of Presentations at the International Conference on Basic and Clinical Multimodal Imaging (BaCI), a Joint Conference of the International Society for Neuroimaging in Psychiatry (ISNIP), the International Society for Functional Source Imaging (ISFSI), the International Society for Bioelectromagnetism (ISBEM), the International Society for Brain Electromagnetic Topography (ISBET), and the EEG and Clinical Neuroscience Society (ECNS), in Geneva, Switzerland, September 5-8, 2013.

Clinical EEG and neuroscience·2013
Same author

Metabolic networks in epilepsy by MR spectroscopic imaging.

Acta neurologica Scandinavica·2012
Same author

Abnormalities of cortical thickness in postictal psychosis.

Epilepsy & behavior : E&B·2011
Same author

Chest Pain: Delays in seeking medical attention.

Canadian family physician Medecin de famille canadien·2011

Altered sodium channel mRNA ratios in epileptic human brain suggest a role in epilepsy pathophysiology. Ligase detection reaction revealed significant changes in sodium channel subtypes 1 and 2 expression.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Epilepsy Research

Background:

  • Neuronal excitability is influenced by voltage-gated ion channels, particularly sodium channels.
  • Aberrant ion channel distribution and density are implicated in neurological disorders like epilepsy.

Purpose of the Study:

  • To investigate the role of sodium channels in epilepsy by quantifying mRNA expression of subtypes 1 and 2.
  • To compare sodium channel mRNA ratios in normal versus epileptic human brain tissues.

Main Methods:

  • Utilized ligase detection reaction (LDR) for precise mRNA quantification.
  • Analyzed relative mRNA quantities of sodium channel subtypes 1 and 2 in human brain samples.

Main Results:

  • Normal human brains displayed consistent regional ratios of sodium channel subtypes 1 and 2 mRNA (approximately 1:2).

Related Experiment Videos

  • Epileptic brain tissues showed significant alterations, up to threefold changes, in these characteristic ratios in corresponding regions.
  • These ratios were invariant among individuals in normal brain samples.

    • Conclusions:

    • The observed significant changes in sodium channel mRNA expression support their involvement in epilepsy.
    • Voltage-gated sodium channels are potential targets for understanding and treating epilepsy pathophysiology.