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[PSI] and [URE3] as yeast prions

R B Wickner1, D C Masison, H K Edskes

  • 1Section on Genetics of Simple Eukaryotes, National Institute of Diabetes, Digestive and Kidney Diseases, Bethesda, MD 20892-0830, USA.

Yeast (Chichester, England)
|December 1, 1995
PubMed
Summary

The yeast genetic elements [URE3] and [PSI] are proposed to be prions, infectious proteins. Evidence includes their curability, dependence on specific genes, and increased acquisition rates with gene overproduction.

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Area of Science:

  • * Genetics
  • * Molecular Biology
  • * Protein Biochemistry

Background:

  • * The non-Mendelian genetic elements [URE3] and [PSI] in yeast mimic chromosomal mutations.
  • * [URE3] affects nitrogen catabolic enzyme expression by interacting with the URE2 gene.
  • * [PSI] enhances readthrough of translational termination, similar to SUP35 gene mutations.

Purpose of the Study:

  • * To propose that [URE3] and [PSI] are prions, infectious proteins.
  • * To provide evidence supporting the prion hypothesis for these yeast elements.
  • * To draw parallels between yeast prions and mammalian transmissible spongiform encephalopathies.

Main Methods:

  • * Analysis of the curability of [URE3] and [PSI] elements.
  • * Investigation of the genetic requirements for [PSI] and [URE3] propagation.

Related Experiment Videos

  • * Examination of the effect of Sup35p and Ure2p overproduction on element acquisition.

    • Main Results:

    • * Both [URE3] and [PSI] were found to be reversibly curable.
    • * Propagation of [PSI] requires the SUP35 gene, and [URE3] requires the URE2 gene.
    • * Overproducing Sup35p or Ure2p increased the frequency of cells acquiring [PSI] or [URE3], respectively.

    Conclusions:

    • * The evidence strongly supports the hypothesis that [URE3] and [PSI] are prions.
    • * These yeast prions share similarities with infectious proteins causing mammalian prion diseases.
    • * The findings contribute to understanding prion biology beyond mammalian systems.