Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mutagenicity studies with palm fruit carotene

M Masuda1, M Haresaku, T Takei

  • 1Human Safety Evaluation Center, Research and Development Headquarters, Lion Corporation, Kanagawa, Japan.

The Journal of Toxicological Sciences
|November 1, 1995
PubMed
Summary

Palm fruit carotene was evaluated for mutagenicity using bacterial, mammalian cell, and mouse tests. The study concluded that palm fruit carotene exhibits no mutagenic activity in these comprehensive in vitro and in vivo assessments.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Study of a rat skin in vivo micronucleus test: data generated by mitomycin C and methyl methanesulfonate.

Mutation research·1999
Same author

Capillary endothelial cell tropism of PVC-211 murine leukemia virus and its application for gene transduction.

Journal of virology·1997
Same author

Platelet endothelial cell adhesion molecule-1 is a major SH-PTP2 binding protein in vascular endothelial cells.

FEBS letters·1997
Same author

A novel gene suppressed in the ventricle of carnitine-deficient juvenile visceral steatosis mice.

FEBS letters·1997
Same author

Retardation of pancreatic regeneration after partial pancreatectomy in a strain of rats without CCK-A receptor gene expression.

Pancreas·1997
Same author

[Seasonal changes of laboratory data in patients with Japanese cedar pollinosis].

Rinsho byori. The Japanese journal of clinical pathology·1997

Area of Science:

  • Toxicology
  • Genetics
  • Carotenoid research

Background:

  • Carotenoids, like palm fruit carotene, are natural compounds with potential health benefits.
  • Assessing the safety profile of food components is crucial for consumer health.
  • Understanding the mutagenic potential of food-derived substances is a key aspect of toxicology.

Purpose of the Study:

  • To evaluate the mutagenicity of palm fruit carotene.
  • To determine if palm fruit carotene can induce genetic mutations or chromosomal damage.

Main Methods:

  • Bacterial reverse mutation assay (Ames test) using Salmonella typhimurium and Escherichia coli strains.
  • In vitro chromosomal aberration test using a Chinese hamster fibroblast cell line (CHL).
  • In vivo micronucleus test in CD-1(ICR) male mice.

Related Experiment Videos

Main Results:

  • Palm fruit carotene did not induce reverse mutations in any of the bacterial strains tested.
  • No structural or numerical chromosomal aberrations were observed in mammalian cells exposed to palm fruit carotene.
  • The in vivo micronucleus test showed no increase in micronucleated polychromatic erythrocytes in mice.

Conclusions:

  • Palm fruit carotene demonstrated no mutagenic activity in the bacterial reverse mutation assay.
  • Palm fruit carotene did not cause chromosomal damage in mammalian cells.
  • The study concludes that palm fruit carotene is not mutagenic based on the conducted in vitro and in vivo assays.