Related Experiment Videos
Expression of renin in coagulating glands
1Department of Animal Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Histology and Histopathology
|January 1, 1996
Summary
Renin in the coagulating gland (CG) is synthesized and released via exocrine secretion, with its production dependent on testosterone. This local renin system
Area of Science:
- Reproductive biology
- Endocrinology
- Molecular biology
Background:
- Local renin-angiotensin systems exist in various tissues, but their functions remain unclear.
- Renin from the coagulating gland (CG) is a recently identified local renin.
- Investigating CG renin requires histological and molecular biological methods.
Purpose of the Study:
- To investigate the presence, localization, and regulation of renin in the coagulating gland (CG) of mice.
- To determine the developmental and hormonal influences on CG renin expression and secretion.
Main Methods:
- Immunohistochemistry to detect renin in CG epithelial cells and excretory products.
- Colloidal gold electron microscopy to visualize renin localization within cellular granules.
- Northern blot analysis and hybridohistochemistry to detect renin mRNA.
Main Results:
- Renin was detected in CG epithelial cells, lysosomal granules, and exocrine granules, secreted via exocytosis.
- Renin immunoreactivity appeared at 6 weeks post-birth, increasing with age.
- Castration reduced renin-containing cells, while testosterone injection increased them.
- Renin mRNA was confirmed in CGs, indicating local synthesis.
Conclusions:
- Testosterone regulates the synthesis of renin in the coagulating gland.
- Renin is released from CGs through an exocrine secretion pathway.
- The coagulating gland possesses a functional, testosterone-dependent local renin system.