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Changes in NADPH diaphorase activity in forebrain structures of the laminae terminalis after chronic dehydration
J Ciriello1, S L Hochstenbach, L Pastor Solano-Flores
1Department of Physiology, University of Western Ontario, London, Canada.
Abstract:
The effect of 3-day chronic dehydration on nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-diaphorase) histochemical staining in forebrain circumventricular structures was investigated in the rat. Increased number and/or intensity of staining of NADPH-diaphorase positive neurons was observed in subfornical organ, organum vasculosum of the laminae terminalis, and median preoptic nucleus. In addition, dense punctate NADPH-diaphorase reaction product was found throughout the internal portion of median eminence. These data suggest the involvement of nitric oxide synthase producing neurons in homeostatic mechanisms controlling body fluid balance and the circulation.