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Trans-kingdom conjugation between Agrobacterium tumefaciens and Saccharomyces cerevisiae, a bacterium and a yeast
Y Sawasaki1, K Inomata, K Yoshida
1Department of Biological Science, Hiroshima University, Japan. 515582/ue.ipc.hiroshima-u.ac.jp
Plant & Cell Physiology
|January 1, 1996
Abstract:
For conjugation between prokaryotic Agrobacterium tumefaciens and eukaryotic Saccharomyces cerevisiae, we constructed two novel conjugative plasmids. A. tumefaciens transmitted the plasmids to S. cerevisiae with the aid of tra genes on a helper plasmid. The transmitted plasmids retained their original structure and function in transconjugant yeasts. The presence of Ti plasmid barely affected the trans-kingdom conjugation.