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Analysis of a short tandem repeat locus on chromosome 19 (D19S253)
F De Stefano1, L Casarino, M G Costa
1Istituto di Medicina Legale, Università degli Studi di Genova, Italy.
International Journal of Legal Medicine
|January 1, 1996
Abstract:
A tetranucleotide tandem repeat locus on chromosome 19 (D19S253) was analysed. PCR products were detected by denaturing polyacrylamide gels with fluorescent-based technology. This study has confirmed a polymorphism with 9 alleles ranging from 209 to 241 bp with a simple repeat structure arranged from 7 to 15 repeats. Family studies confirmed mendelian inheritance of alleles. The efficiency on DNA extracted from bloodstains and cigarette butts has been evaluated. The protocol has shown sensitivity and reproducibility.