High-resolution vertical PAGE: an alternative electrophoretic system with multiple forensic applications

DNA profiling based on PCR technology has become a powerful tool in forensic casework, as it enables specific amplification of polymorphic VNTR loci from very small quantities or even degraded human DNA. Genetic typing of AmpFLP or STR loci may require electrophoretic separation techniques usually achieved by sequencing gels. In this report we present a non-denaturing high-resolution vertical PAGE system which can be easily used for both VNTR subgroups. The system has been evaluated for single- and multiplex use in routine casework and has been shown to be rapid, sensitivite and reproducible.