Diagnosis of Helicobacter pylori infection

A number of invasive and non-invasive techniques are available for the diagnosis of infection with Helicobacter pylori. These can be used to establish whether infection is present and to confirm that eradication has been achieved after treatment. Invasive techniques require biopsy of the gastric mucosa; such techniques include histology, bacterial culture, the rapid urease test and the polymerase chain reaction (PCR) technique. The principal non-invasive techniques are the 13C-urea breath test and serological assays in serum or saliva samples. Histological examination of gastric biopsy samples should be mandatory at the initial presentation of the patient because it also gives insight on the status of the gastric mucosa. The rapid urease test can be used to give a rapid diagnosis of infection. Eradication should also be confirmed by histological examination, though other sensitive tests such as bacterial culture, PCR or the 13C-urea breath test may also be used.