Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Orientation dependence in homologous recombination

K Yamamoto1, N Takahashi, Y Fujitani

  • 1Laboratory of Clinical Microbiology and Immunology, Bun 'in Hospital, Faculty of Medicine, University of Tokyo, Japan. backen@camille.is.s.u-tokyo.ac.jp

Genetics
|May 1, 1996
PubMed
Summary

Homologous recombination in Escherichia coli differs between recBC sbcBC and recBC sbcA strains. Recombination efficiency depends on repeat orientation in recBC sbcBC strains but not recBC sbcA strains.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cell culture systems for the detection of HCV infection.

Methods in molecular medicine·2011
Same author

Detection of a 5' end subgenome of hepatitis C virus terminating at nucleotide 384 in patients' plasma and liver tissues.

Journal of viral hepatitis·2006
Same author

Identification of new parvovirus DNA sequence in swine sera from Myanmar.

Japanese journal of infectious diseases·2002
Same author

Cross-clade neutralizing antibody production against human immunodeficiency virus type 1 clade E and B' strains by recombinant Mycobacterium bovis BCG-based candidate vaccine.

Vaccine·2001
Same author

RNAse A treatment of Taq and Tth DNA polymerases eliminates primer/template-independent poly(dA-dT) synthesis.

BioTechniques·2001
Same author

Institute profile: National Institute of Infectious Diseases.

Trends in microbiology·2001

Area of Science:

  • Molecular Biology
  • Genetics
  • Microbiology

Background:

  • Homologous recombination is a fundamental genetic process essential for DNA repair and genome stability.
  • Understanding recombination mechanisms in bacteria like Escherichia coli provides insights into broader biological principles.

Purpose of the Study:

  • To investigate the influence of repeat orientation (direct vs. inverted) on homologous recombination efficiency in different Escherichia coli strains.
  • To compare recombination outcomes in recBC sbcBC and recBC sbcA mutant strains.
  • To determine the effect of double-strand breaks on recombination rates.

Main Methods:

  • Utilized two plasmids with defective neo genes in direct or inverted orientations.
  • Measured recombination frequency in recBC sbcBC and recBC sbcA strains.

Related Experiment Videos

  • Employed fluctuation tests and direct selection assays, with and without induced double-strand breaks.

    • Main Results:

    • Recombination between direct repeats was significantly higher than inverted repeats in recBC sbcBC strains.
    • Recombination efficiency was similar for both direct and inverted repeats in recBC sbcA strains.
    • Double-strand breaks consistently elevated recombination rates across both strains and repeat orientations.

    Conclusions:

    • The orientation of homologous repeats critically affects recombination in recBC sbcBC strains, unlike recBC sbcA strains.
    • These findings support distinct major recombination pathways in recBC sbcBC and recBC sbcA Escherichia coli strains.
    • Double-strand breaks act as a potent enhancer of homologous recombination in this system.