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Related Experiment Videos

Large, complex modular structure of a fission yeast DNA replication origin

D D Dubey1, S M Kim, I T Todorov

  • 1Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Buffalo, New York 14263, USA.

Current Biology : CB
|April 1, 1996
PubMed
Summary

Fission yeast DNA replication origins share a modular structure with budding yeast origins, but are larger and more complex. This suggests increased protein-DNA interactions are required for origin function in Schizosaccharomyces pombe.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Yeast Biology

Background:

  • DNA replication origins in Saccharomyces cerevisiae (budding yeast) are linked to autonomously replicating sequence (ARS) elements.
  • These ARS elements feature an ARS consensus sequence (ACS) and smaller stimulatory motifs within ~150 bp.
  • The structure of replication origins in the evolutionarily distant Schizosaccharomyces pombe (fission yeast) remains less understood.

Purpose of the Study:

  • To investigate the sequence requirements for the function of the Schizosaccharomyces pombe chromosomal replication origin, ars3002.
  • To determine if S. pombe replication origins share structural similarities with those of S. cerevisiae.

Main Methods:

  • Deletion analysis of the ars3002 region.
  • Linker substitution scanning to identify critical sequences.

Related Experiment Videos

  • Functional assessment of sequence elements in ars3002.

    • Main Results:

    • Two large essential regions (30-55 bp) and additional stimulatory sequences were identified within a 600 bp fragment of ars3002.
    • These essential regions are similar to each other and to sequences found in other S. pombe ARS elements.
    • The identified essential regions may function as the S. pombe equivalent of the S. cerevisiae ACS.

    Conclusions:

    • The S. pombe replication origin ars3002 exhibits a modular structure, similar to S. cerevisiae origins.
    • However, ars3002 is larger and possesses more and larger modules compared to S. cerevisiae origins.
    • This suggests that replication origin function in S. pombe necessitates a greater number of protein-DNA interactions than in S. cerevisiae.