[Comparative study of the kinetics of phospholipid liposome peroxidation, induced by hypochlorite and in the Fe2+ +...

The present study examined the kinetics of accumulation of lipid peroxidation products in phosphatidylcholine liposomes incubated with hypochlorite (HOCl/OCl-) or with FeSO4 in the presence of ascorbate. The incubation of liposomes with HOCl/OCl- led to the accumulation of primary (lipid hydroperoxides and conjugated dienes) and secondary (TBA-reactive substances) lipid peroxidation products identical to those generated in the presence of Fe2+/ascorbate. However the rate of the formation of lipid peroxidation products as well as the gain in their amount were sufficiently higher (4-150-fold and 3-6-fold, respectively) in the case of HOCl/OCl(-)-induced lipid peroxidation as compared to lipid peroxidation in the Fe2+/ascorbate system. The accumulation of lipid peroxidation products lasted until HOCl/OCl- was present in the reaction mixture. The data obtained support our hypothesis that hypochlorite produced by activated neutrophils and monocyte/macrophages may be reasonable for the initiation of lipid peroxidation in blood.