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An Improved Method of RNA Isolation from Loblolly Pine (P. taeda L.) and Other Conifer Species
Published on: February 22, 2010
An improved method for preparing the chromosomes of pines and other gymnosperms
1School of Biological Sciences, University of Auckland, New Zealand.
Abstract:
A simple technic is described to produce well spread gymnosperm chromosomes. Root tip meristems are digested with a pectinase:cellulase mixture to produce a cell suspension which then is squashed to yield flat, well spread chromosome complements that can be stained or used for in situ hybridization.
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