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Accumulation of NO synthase (type-I) at the neuromuscular junctions in adult mice
L Oliver1, O Goureau, Y Courtois
1Centre de Gérontologie, Institut National de la Santé et de la Recherche Médicale, Association Claude-Bernard, Paris, France.
Abstract:
Recent data have shown that NOS-I is localized almost exclusively to the sarcolemma of fast-twitch fibers, where it probably interacts with the dystrophin-glycoprotein complex. The concentration of dystrophin-related protein at the neuromuscular junctions and the possible involvement of NO in synaptic suppression led us to investigate the presence of NOS-I at the adult motor endplates. Our data clearly show that NOS-I protein, detected by immunohistochemistry accumulates at the adult endplates. Furthermore, the absence of NOS-I protein at the denervated neuromuscular junctions suggest a neural origin of this enzyme. The putative roles of NO at the endplate are discussed.