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The relationship of Piry virus to other vesiculoviruses: a re-evaluation based on the glycoprotein gene sequence
Abstract:
By sequencing the 3 half of the Piry virus genome, we show that Piry virus, like the other vesiculoviruses, contains the genes for nucleoprotein N, phosphoprotein P, matrix protein M, glycoprotein G and polymerase protein L, in that order. Our analysis of the Piry G protein sequence suggests that Piry and Chandipura are related to each other as closely as the Indiana and New Jersey vesicular stomatitis virus serotypes are to each other. A re-examination of amino acid sequences in the nucleocapsid protein shows that this relationship is also true of the more conserved central region of this protein and that the greatest divergence between Piry and Chandipura has occurred in two other regions of the nucleocapsid protein.
Insights
Piry virus genome sequencing reveals its gene order, similar to other vesiculoviruses. This study clarifies the close evolutionary relationship between Piry and Chandipura viruses.
Area of Science:
- Virology
- Molecular Biology
- Genomics
Background:
- Vesiculoviruses are a genus of RNA viruses known for causing significant diseases in animals.
- Understanding the genetic makeup and evolutionary relationships of these viruses is crucial for disease control and prevention.
Purpose of the Study:
- To sequence the 3 half of the Piry virus genome.
- To determine the gene order of Piry virus.
- To analyze the evolutionary relationship between Piry virus and other vesiculoviruses, specifically Chandipura virus.
Main Methods:
- Genome sequencing of the Piry virus.
- Bioinformatic analysis of viral protein sequences, including glycoprotein G and nucleoprotein N.
Main Results:
- The Piry virus genome contains genes for nucleoprotein (N), phosphoprotein (P), matrix protein (M), glycoprotein (G), and polymerase (L) in a conserved order.
- Sequence analysis of the G protein indicates a close evolutionary relationship between Piry and Chandipura viruses, comparable to that between Indiana and New Jersey serotypes of vesicular stomatitis virus.
- Re-examination of nucleocapsid protein sequences confirms this close relationship, with divergence concentrated in specific regions.
Conclusions:
- Piry virus shares conserved genomic features with other vesiculoviruses.
- Piry and Chandipura viruses are closely related, with specific regions of the nucleocapsid protein showing the most divergence.
- The findings contribute to a better understanding of vesiculovirus evolution and taxonomy.