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Destabilization of simple repetitive DNA sequences by transcription in yeast
M Wierdl1, C N Greene, A Datta
1Department of Biology, University of North Carolina, Chapel Hill 27599-3280, USA.
Genetics
|June 1, 1996
Abstract:
Simple repetitive DNA sequences in the eukaryotic genome frequently alter in length. In wild-type strains, we find that transcription through a repetitive poly GT tract destabilizes the tract four- to ninefold. In mismatch repair-deficient yeast strains, simple repeats are very unstable. High levels of transcription in such strains destabilize repetitive tracts an additional two- to threefold.