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Related Experiment Videos

Exocytosis: proteins and perturbations

G J Augustine1, M E Burns, W M DeBello

  • 1Department of Neurobiology, Duke University Medical Center, Durham, North Carolina 27710, USA.

Annual Review of Pharmacology and Toxicology
|January 1, 1996
PubMed
Summary

Cellular secretion relies on exocytosis, a process involving membrane fusion. This review explores how perturbing protein function in living cells reveals the roles of proteins in exocytosis.

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Area of Science:

  • Cell Biology
  • Molecular Biology

Background:

  • Exocytosis is the fundamental process for cellular secretion, involving the fusion of secretory vesicles with the plasma membrane.
  • While many proteins are known to be involved in secretory cells, their specific roles in mediating exocytosis remain largely undefined.

Purpose of the Study:

  • To review strategies for perturbing protein function in living secretory cells.
  • To elucidate the functional roles of proteins in the complex process of exocytosis.

Main Methods:

  • Review of various protein perturbation strategies.
  • Analysis of studies that have manipulated protein function in live secretory cells.

Main Results:

  • Perturbation strategies have provided crucial insights into the functions of specific proteins during exocytosis.

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  • Understanding protein dynamics is key to deciphering the exocytotic mechanism.

    • Conclusions:

    • Perturbing protein function is essential for assigning unambiguous roles to proteins in exocytosis.
    • A molecular model of protein dynamics during exocytosis has been developed based on these findings.